Honda T, Nakayama R, Kawahara Y, Yuge L, Ueda K. Kaposi’s sarcoma-associated herpesvirus is cell-intrinsically controlled in latency in microgravity. Virus Res 276: 197821, 2020. https://pubmed.ncbi.nlm.nih.gov/31756357/
Imura T, Otsuka T, Kawahara Y, Yuge L. “Microgravity” as a unique and useful stem cell culture environment for cell-based therapy. Regen Ther 12: 2-5, 2019. https://pubmed.ncbi.nlm.nih.gov/31890760/
Koaykul C, Kim MH, Kawahara Y, Yuge L, Kino-Oka M. Maintenance of neurogenic differentiation potential in passaged bone marrow-derived human mesenchymal stem cells under simulated microgravity conditions. Stem Cells Dev 28: 1552-1561, 2019. https://pubmed.ncbi.nlm.nih.gov/31588849/
Kurose T, Takahashi S, Otsuka T, Nakagawa K, Imura T, Sueda T, Yuge L. Simulated microgravity-cultured mesenchymal stem cells improve recovery following spinal cord ischemia in rats. Stem Cell Res 41: 101601, 2019. https://pubmed.ncbi.nlm.nih.gov/31731179/
Koaykul C, Kim MH, Kawahara Y, Yuge L, Kino-Oka M. Alterations in nuclear lamina and the cytoskeleton of bone marrow-Derived human mesenchymal stem cells cultured under simulated microgravity conditions. Stem Cells Dev 28: 1167-1176, 2019. https://pubmed.ncbi.nlm.nih.gov/31169056/
Fukazawa T, Tanimoto K, Shrestha L, Imura T, Takahashi S, Sueda T, Hirohashi N, Hiyama E, Yuge L.Simulated microgravity enhances CDDP-induced apoptosis signal via p53-independent mechanisms in cancer cells. PLoS One 14: e0219363, 2019. https://pubmed.ncbi.nlm.nih.gov/31323026/
Otsuka T, Imura T, Nakagawa K, Shrestha L, Takahashi S, Kawahara Y, Sueda T, Kurisu K, Yuge L. Simulated Microgravity Culture Enhances the Neuroprotective Effects of Human Cranial Bone-Derived Mesenchymal Stem Cells in Traumatic Brain Injury. Stem Cells Dev 27: 1287-1297, 2018. https://pubmed.ncbi.nlm.nih.gov/29790427/
Imura T, Nakagawa K, Kawahara Y, Yuge L. Stem Cell Culture in Microgravity and Its Application in Cell-Based Therapy. Stem Cells Dev 27: 1298-1302, 2018. https://pubmed.ncbi.nlm.nih.gov/29978759/
Furukawa T, Tanimoto K, Fukazawa T, Imura T, Kawahara Y, Yuge L. Simulated microgravity attenuates myogenic differentiation via epigenetic regulations. npj Microgravity, 4: 11, 2018. https://pubmed.ncbi.nlm.nih.gov/29845109/
Mitsuhara T., Takeda M., Yamaguchi S., Manabe T., Matsumoto M., Kawahara Y., Yuge L., Kurisu K.: Simulated microgravity facilitate cell migration and neuroprotection after bone marrow stromal cell transplantation in spinal cord injury. Stem Cell Research & Therapy, 4: 35, 2013. https://pubmed.ncbi.nlm.nih.gov/23548163/
Yuge L., Sasaki A,, Kawahara Y., Wu SL., Matsumoto M., Manabe T., Kajiume T., Takeda M., Magaki T., Takahashi T., Kurisu K., Matsumoto M.: Simulated microgravity maintains the undifferentiated state and enhances the neural repair potential of bone marrow stromal cells. Stem Cells Dev 20: 893-900, 2011. https://pubmed.ncbi.nlm.nih.gov/20828292/
Wakayama S., Kawahara Y., Li C., Yamagata K., Yuge L., WakayamaT.: Detrimental effects of microgravity on mouse preimplantation development in vitro. PLoS ONE 4: e6753, 2009. https://pubmed.ncbi.nlm.nih.gov/19707597/
Takeda M., Magaki T., Okazaki T., Kawahara Y., Manabe T., Yuge L., Kurisu K.: Effects of simulated microgravity on proliferation and chemosensitivity in malignant glioma cells. Neurosci Lett. 463: 54-59, 2009. https://pubmed.ncbi.nlm.nih.gov/19628020/
Kawahara Y., Manabe T., Matsumoto M., Kajiume T., Matsumoto M., Yuge L.: LIF-free embryonic stem cell culture in simulated microgravity. PLoS ONE 4: e6343, 2009. https://pubmed.ncbi.nlm.nih.gov/19626124/
Makihira S., Kawahara Y., Yuge L., Mine Y., Nikawa H.: Impact of the microgravity environment in a three-dimensional clinostat on osteoblast- and osteoclast-like cells. Cell Biol Int 32: 1176-1181, 2008. https://pubmed.ncbi.nlm.nih.gov/18550393/
Yuge L., Kajiume T., Tahara H., Kawahara Y., Umeda C., Yoshimoto R., Wu S.L., Yawaoka K., Asashima M., Kataoka K., Ide T.: Microgravity potentiates stem cell proliferation while sustaining the capability of differentiation. Stem Cells Dev, 15: 921-929, 2006. https://pubmed.ncbi.nlm.nih.gov/17253953/
Hirasaka K., Nikawa T., Yuge L., Ishihara I., Higashibata A., Ishioka N, Okubo A., Miyashita T., Suzue N., Ogawa T., Oarada M., Kishi K.: Clinorotation prevents differentiation of rat myoblastic L6 cells in association with reduced NF-<kappa>B-signaling. BBA-Mol Cell Res 1743: 130-140, 2005. https://pubmed.ncbi.nlm.nih.gov/15777848/
Yuge L., Hide I., Kumagai T., Kumei Y., Takeda S., Kanno M., Sugiyama M., Kataoka K.: Cell differentiation and p38MAPK cascade are inhibited in human osteoblasts cultured in a 3D-clinostat. In Vitro Cell Dev Biol-Animal 39: 89-97, 2003. https://pubmed.ncbi.nlm.nih.gov/12892532/
Kawahara Y., Yuge L.: Simulated microgravity based stem cell cultures enhance their utility for cell-based therapy. Current Biotechnology, 2, 2013.
弓削 類: 重力が生体に与える影響. 理学療法, 26: 585-589, 2009.
学会発表
シンポジウム・招待講演(海外)
Yuge L: The Effect of Different Gravity Environments at the Cellular Level. 6th Annual Neuroscience-20, Osaka, 2019年6月28日.
Yuge L.: Microgravity Facilitates Stem Cell Proliferation and Neural Differentiation after Cell-based Therapy.14th Society for Brain Mapping and Therapeutics (SBMT), Los Angeles, 2017年4月18日.
Yuge L.: Microgravity Facilitates Stem Cell Proliferation and Neural Differentiation after Cell Transplantation in Neurological Disorder Models. 10th International Society for Stem Cell Research (ISSCR), Yokohama, Kanagawa, June 15, 2012
Yuge L.: Microgravity facilitates stem cell proliferation and neural differentiation after transplantation. 6th Annual World Congress for Brain Mapping and Image Guided Therapy-International Brain Mapping and Intraoperative Surgical planning Sossiety (IBMISPS), Harvard Medical School, Boston, USA, August 28, 2009 (Scientific Session 13: Rehabilitation Medicine: Stem Cell/Chair: Louis Yuge DrMedSc, PhD)
Yuge L.: Stem cell culture in microgravity. BIT’s 1st Annual World Congress of Regenerative Medicine & Stem Cell 2008, Sanshui Garden Hotel (FSGH), Foshan, China, December 2, 2008 (Session2: Bioreactors and cell mechanics for tissue engineering, and angiogenesis in rehenerative medicine)
Yuge L.: Stem cell culture in microgravity. 12th ADNAT convention, Symposium on Biology of Embryonic and Adult Stem Cells, Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India, February 24, 2008
Otsuka T, Imura T, Nakagawa K, Kurose T, Teranishi M, Karim Md. S, KawaharaY, Kurisu K, Yuge L. Simulated microgravity culture potentiates neuroprotective effect of mesenchymal stem cells. 35th Annual Meeting of the American Society for Gravitational and Space Research (ASGSR), Denver, CO, USA, 2019年11月20日
Yuge L: Endeavor of regenerative medicine combined with cellular based therapy using microgravity and robotic rehabilitation. 5th Tissue Engineering and Regenerative Medicine International Society (TERMIS), Kyoto, 2018年9月7日.
Kim MH, Koaykul C, Kawahara Y, Yuge L, Kino-oka M: Study of human mesenchymal stem cell behaviors on isotropic gravity. 5th Tissue Engineering and Regenerative Medicine International Society (TERMIS), Kyoto, 2018年9月7日.
Yuge L, Otsuka T, Imura T, Nakagawa K, Shrethta L, Kawahara Y, Abiko M, Oshita J, Kurisu K :Simulated microgravity culture increases the neuroprotective potential of mesenchymal stem cells. 33rd American Society for Gravitational and Space Research (ASGSR), Seatle, 2017年10月26日
Imura T, Otsuka T, Nakagawa K.,Shrestha L, Kawahara Y, Abiko M, Oshita J, Kurisu K, Yuge L,: Simulated microgravity culture increases the neuroprotective potential of human cranial bone derived mesenchymal stem cells in brain. 15th International Society for Stem Cells Research (ISSCR), Boston, 2017年6月16日.
Yuge L, Otsuka T, Imura T, Nakagawa K.,Shrestha L, Kawahara Y, Abiko M, Oshita J, Kurisu K, Yuge L,: Cranial bone derived mesenchymal stem cells culterd in simulated microgravity have highly neuroprotective potential against stress induced NG-108 cell death in vitro. 15th International Society for Stem Cells Research (ISSCR), Boston, 2017年6月16日.
Nakagawa K., Imura T., Otsuka T., Shrestha L., Shinagawa K., Takahashi S., Sueda T., Kurisu K., Kawahara Y., Yuge L.: Ther effects of simulated microgravity on neural differentiation of human cranial bone marrow-derived mesenchymal stem cells. 32nd American Society for Gravitational and Space Research (ASGSR) Cleveland, OH, USA, October 26, 2016.
Kawahara Y., Furukawa T., Fukazawa T., Tanimoto K., Ookura Y., Tomiyasu M., Otsuru N., Yuge L.: Skeletal muscle derived myoblast differentiation inhibitory mechanism in simulated microgravity environment. 13th International Society for Stem Cell Research (ISSCR), Stockholm, Sweden, June 24, 2015
Kawahara Y., Furukawa T, Fukazawa T., Tanimoto K., Otsuru N., Imura T., Takahashi S., Sueda T., Kurisu K., Kumei Y., Yuge L.:Epigenetic regulation of the myoblast differentiation under simulated microgravity. 31th Annual Meeting of the American Society for Gravitational and Space Research(ASGSR), Alexandria, Virginia November 11 2015
Kawahara Y., Nakata K., Fukazawa T., Tanimoto K., Furukawa T., Ookura Y., Otsuru N., Yuge L.: Human mesenchymal stem cells differentiation to chondrocyte using simulated microgravity environment. 12th International Society for Stem Cell Research (ISSCR), Vancouver, Canada, June 19, 2014
Fukazawa T., Tanimoto K., Furukawa T., Ookura Y., Otsuru N., Kawahara Y., Yuge L.: Simulated microgravity modifies CDDP sensitivity of cancer cells possibly through the p53 pathway. 12th International Society for Stem Cell Research (ISSCR), Vancouver, Canada, June 19, 2014
Yuge L., Nakata K., Fukazawa T., Tanimoto K., Furukawa T., Ookura Y., Tomiyasu M., Otsuru N., Kawahara Y.: Simulated microgravity inhibits chondrocyte differentiation on human mesenchymal stem cells. 30th Annual Meeting of the American Society for Gravitational and Space Research (ASGSR), Pasadena, CA, USA, October 23, 20144
Kawahara Y., Fukazawa T., Furukawa T., Ookura Y., Tomiyasu M., Otsuru N., Yuge L.: Embryonic stem cell culture and induced pluripotent stem cell in simulated microgravity environment. 30th Annual Meeting of the American Society for Gravitational and Space Research (ASGSR), Pasadena, CA, USA, October 23, 2014
Kawahara Y., Morikawa K., Matsumoto M., Fukazawa T., Imura T., Khalesi E., Sun YN., Uwatoko H., Nakata K., Ookura Y., Furukawa T., Yuge L.: Gravity environment changing is a key factor for muscle differentiation regulation. 11st International Society for Stem Cell Research (ISSCR), Boston, MA, USA, June 13, 2013
Kawahara Y., Matsumoto M., Fukazawa T., Imura T., Khalesi E., Morikawa K., Sun YN., Uwatoko H., Nakata K., Tanimoto K., Yuge L.: Simulated microgravity initiates a change in cytoskeleton to proliferate human mesenchymal stem cells. 10th International Society for Stem Cell Research (ISSCR), Yokohama, Kanagawa, June 14, 2012
Kawahara Y., Matsumoto M., Fukazawa T., Imura T., Khalesi E., Sun YN., Uwatoko H., Nakata K., Yuge L.: Simulated microgravity induces indirectly cytoskeleton changes to proliferate human mesenchymal stem cells. 8th World Stem Cell Summit, West Palm Beach, FL, USA, December 3, 2012
Kawahara Y., Takeda M., Mitsuhara T., Manabe T., Matsumoto M., Takahashi T., Kurisu K., Matsumoto M., Yuge L.: Simulated microgravity increases cell migration and neuroprotective effect after bone marrow stromal cell transplantation spinal cord injury. 9th International Society for Stem Cell Research (ISSCR), Toronto, Canada, June 17, 2011
Kawahara Y., Kajiume T., Matsumoto M., Fukazawa T., Imura T., Morikawa K., Matsumoto M., Yuge L.: Simulated microgravity makes mouse embryonic stem cells aggregate to maintain pluripotency in LIF-free culture. BIT’s 3rd Annual World Congress of Regenerative Medicine & Stem Cells (RMSC) 2010, Everbright Convention & Exhibition Center, Shanghai, China, December 5-7, 2010
Morikawa K., Matsumoto M., Fukazawa T., Imura T., Sun YN., Khalesi E., Kawahara Y., Yuge L.:Gravity affects myoblast differentiation and undifferentiation -Myotub developmen study-. BIT’s 3rd Annual World Congress of Regenerative Medicine & Stem Cells (RMSC) 2010, Everbright Convention & Exhibition Center, Shanghai, China, December 5-7, 2010
Kawahara Y., Kajiume T., Matsumoto M. Imura T., Matsumoto M., Yuge L.: A specific-gravity medium: novel approach for mouse hematopoietic stem cell culture. 8th International Society for Stem Cell Research (ISSCR), San Francisco, CA, USA, June 18, 2010
Imura T., Matsumoto M., Kawahara Y., Yuge L.: Aggregates formation of mouse ES cells using simulated microgravity and neural differentiation. 8th International Society for Stem Cell Research (ISSCR), San Francisco, CA, USA, June 17, 2010
Wakayama S., Kawahara Y., Li C., Yamagata K., Yuge L., Wakayama T.: Detrimental effects of microgravity on mouse preimplantation development in vitro. 6th Annual Conference of Asian Reproductive Biotechnology Society (ARBS), Seam Reap City, Cambodia, November 16-20, 2009
Kawahara Y., Manabe T., Matsumoto M, Imura T., Kajiume T., Takeda M., Matsumoto M., Yuge L.: Simulated microgravity: a novel approach to embryonic stem cell culture. American Society for Gravitational and Space Biology (ASGSB 25th Annual Meeting NASA), Raleigh, NC, USA, November 7, 2009
Takeda M., Magaki T., Sasaki A., Manabe T., Matsumoto M., Kawahara Y., Yuge L., Kurisu K.: Transplantation of bone marrow stromal cells cultured under simulated microgravity into a spinal cord injury rat. American Society for Gravitational and Space Biology (ASGSB 25th Annual Meeting NASA), Raleigh, NC, USA, November 6, 2009
Kawahara Y., Manabe T., Matsumoto M., Ogawa K., Kajiume T., Takeda M., Magaki T., Yamashita H., Takahashi T., Aoki S., Sueda Y., Matsumoto M., Yuge L.: Simulated microgravity enables LIF-free culture in mouse embryonic stem cells. 7th International Society for Stem Cell Research (ISSCR), Barcelona, Spain, July 9, 2009
Kawahara Y., Manabe T., Ogawa K., Matsumoto M., Yamashita H., Takahashi T., Aoki S., Sueda Y., Matsumoto M., Yuge L.: Microgravity environment is a novel control approach for myoblast differentiation. BIT’s 1st Annual World Congress of Regenerative Medicine & Stem Cell 2008, Sanshui Garden Hotel (FSGH), Foshan, China, December 2-4, 2008
Yuge L., Kawahara Y., Yoshimoto R., Sasaki A., Wu S.L., Manabe T., Ogawa K., Matsumoto M., Kajiume T., Takeda M., Magaki T.: Gravity affects to myoblast differentiation. The ESA ISGP ASGSB ELGRA Meeting “Life in Space for Life on Earth”e for Life on Earth” (29th Annual ISGP Meeting, 10th ESA Life Sciences Symposium, 24th Annual ASGSB Meeting, ELGRA Symposium), Anger, France, June 25, 2008
Takeda M., Okazaki T., Magaki T., Kawahara Y., Yuge L., Kurisu K.: Simulated microgravity inhibits proliferation of glioblastoma cells. The ESA ISGP ASGSB ELGRA Meeting “Life in Space for Life on Earth”e for Life on Earth” (29th Annual ISGP Meeting, 10th ESA Life Sciences Symposium, 24th Annual ASGSB Meeting, ELGRA Symposium), Anger, France, June 26, 2008
Yuge L., Sasaki A., Takeda M., Magaki T., Wu SL., Kawahara Y., Yoshimoto R., Manabe T., Ogawa K., Matsumoto M., Kajiume T.: Microgravity inhibits neural differentiation while sustaining the capability of differentiation. 6th International Society for Stem Cell Research (ISSCR), Philadelphia, PA, USA, June 11, 2008
Takeda M., Okazaki T., Magaki T., Sasaki A., Wu S.L., Kawahara Y., Yoshimoto R., Manabe T., Kajiume T., Yuge L., Kurisu K.: Simulated microgravity inhibits proliferation of malignant glioma cells. American Society for Gravitational and Space Biology (ASGSB 23rd Annual Meeting NASA), Ames Research Center, Moffett Field, CA., USA, October 26, 2007
Yoshimoto R., Kajiume T., Kawahara Y., Sasaki A., Wu S.L., Manabe T., Takeda M., Magaki T., Yuge L.: Characterization of hematopoietic stem cells cultured in 3D-clinostat. American Society for Gravitational and Space Biology (ASGSB 23rd Annual Meeting NASA), Ames Research Center, Moffett Field, CA., USA, October 26, 2007
Sasaki A., Takeda M., Magaki T., Wu S.L., Kawahara Y., Yoshimoto R., Manabe T., Boulenouar A., Kajiume T., Yuge L.: Microgravity inhibits neural differentiation while sustaining the capability of differentiation. American Society for Gravitational and Space Biology (ASGSB 23rd Annual Meeting NASA), Ames Research Center, Moffett Field, CA., USA, October 26, 2007
Kawahara Y., Yoshimoto R., Sasaki A., Wu S.L., Manabe T., Kajiume T., Takeda M., Magaki T., Yuge L.: The differentiation of myoblast is regulated by gravity. American Society for Gravitational and Space Biology (ASGSB 23rd Annual Meeting NASA), Ames Research Center, Moffett Field, CA., USA, October 27, 2007
Umeda C., Nitta J., Kawahara Y., Yoshimoto R., Kajiume T., Sasaki A., Wu S.L., Manabe T., Kanno M., Naminohira K., Nakagawa K., Fushimi T., Fujimura M., Yuge L.: Differentiation of human mesenchymal stem cells and effect of three-dimensional scaffold culture. 15th International Congress of WCPT, Vancouver, Canada, June 5, 2007
Umeda C., Kawahara Y., Yoshimoto R., Kajiume T., Sasaki A., Wu S.L., Naminohira K., Yuge L.: Cytoskeleton changes in mesenchymal stem cells differentiatie to osteoblasts and chondrocytes and effect of electric stimulation during three-dimensional scsffold culture. American Society for Gravitational and Space Biology (ASGSB 22nd Annual Meeting NASA), Washington D.C., USA, November 3, 2006
Yoshimoto R., Kajiume T., Kawahara Y., Umeda C., Sasaki A., Wu S.L., Naminohira K., Yuge L.: Proliferation of hematopoietic stem cells is stimulated in 3D-clinostat culture. American Society for Gravitational and Space Biology (ASGSB 22nd Annual Meeting NASA), Washington D.C., USA, November 3, 2006
Takeda M., Okazaki T., Magaki T., Sasaki A., Wu S.L., Kawahara Y., Umeda C., Yoshimoto R., Kajiume T., Yuge L., Kurisu K. : Simulated microgravity inhibits neural differentiation of mouse bone marrow cells. American Society for Gravitational and Space Biology (ASGSB 22nd Annual Meeting NASA), Washington D.C., USA, November 3, 2006
Yuge L., Kawahara Y., Umeda C., Yoshimoto R., Tahara H., Kajiume T., Ide T., Kataoka K.: Human mesenchymal stem cell proliferation and differentiation using a 3D-clinostat culture. American Society for Gravitational and Space Biology (ASGSB 21st Annual Meeting NASA), Reno, USA, November 3, 2005
Yuge L., Kawahara Y., Umeda C.,Tahara H., Kajiume T., Asashima M., Ide T., Kataoka K. & Kanno M.: Simulated microgravity facilitates human mesenchymal stem cell proliferation and chondrocyte differentiation after transplantation. American Society for Gravitational and Space Biology (ASGSB 20th Annual Meeting NASA), New York, USA, November 12, 2004
Hirasaka K., Yuge L., Ishihara I., Kano M., Asanoma Y., Okubo A., Miyashita T., Ogawa T., Oarada M., Kishi K., Nikawa T.: Clinorotation prevents differentiation of rat myoblastic L6 cells in association with reduced I<kappa>B-ubiqutination. American Society for Gravitational and Space Biology (ASGSB 19th Annual Meeting NASA) Huntsville USA, November 13, 2003
Yuge L., Okubo A., Miyashita T., Kanno M., Kataoka K.: Human mesenchymal stem cells differentiate to chondrocytes cultured in a simulated microgravity. American Society for Gravitational and Space Biology (ASGSB 18th Annual meeting NASA) Cape Canaveral USA, November 8, 2002
Yuge L., Kumei Y., Kanno M., Ikuta Y., Kataoka K., Kajihara H.: Culture in 3D-clinostat inhibits TGF-beta, p38 MAPK in differentiation of human osteoblasts. American Society for Gravitational and Space Biology (ASGSB 17th Annual meeting NASA), Alexandria USA, November 7, 2001